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. 2012 Aug 17;26(11):1917–1927. doi: 10.1210/me.2012-1152

Fig. 4.

Fig. 4.

IL1β treatment reduces HNF-4α mRNA and protein levels after 2–4 h via c-Jun activation. A, Analysis of SHBG and HNF-4α mRNA levels in HepG2 cells treated with vehicle or IL1β (100 ng/ml) at 0, 2, and 4 h. Human 18S (h18S) mRNA was amplified as a control. Data points are shown as mean ± sd of triplicates. **, P < 0.01 compared with the control. B, Western blotting of HNF-4α, phospho-c-Jun, and PPIA in total cell protein extracts of HepG2 cells treated as in A. C, Western blotting of HNF-4α and PPIA in total cell protein extracts of HepG2 cells treated with vehicle, IL1β (100 ng/ml) alone, or IL1β (50 ng/ml) with MG132 (10 μm).