Fasting decreases CART genes expression. A, Effects of 3 d fasting on CART expression in adult zebrafish brain showing a decrease of all CART genes. The data represent mean ± sem values from three zebrafish brains. Fasting decreases CART3 expression specifically in the hypothalamus (K) and the medulla oblongata (E–G, M), whereas expression in the tegmentum remains unaffected by fasting (F, G, and L). B, C, E, and F, Lateral views; D, and G, dorsal views; H–M, transverse sections. *, P < 0.05; **, P < 0.01.