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The proteasomes regulate CRH transcription in cytotrophoblasts. Human cytotrophoblasts were incubated with vehicle (lane 1) or proteasomal inhibitors MG132 (lanes 2–5), lactacystin (lanes 6–9), PSI (lanes 10–13) or nonproteasomal inhibitor, or ZLL (lanes 14–17) at a concentration ranging from 0.1 to 3.0 μm as indicated for 24 h. The total RNA were extracted and subjected to Northern blotting analysis with the use of GAPDH as the reference.
