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sox4b knockdown decreases tshβ, gsuα, and gh expression in 48-hpf embryos. Fertilized eggs were microinjected with sox4bMO and analyzed by WISH for pituitary hormone expression. A–C and G–I, Control embryos; D–F and J–L, sox4b morphants, ventral views (anterior to the top); both tshβ (D) and gsuα (E) expression is almost completely lost, whereas gh expression is decreased (F) and slβ, prl, and pomc are unchanged (J–L). Scale bars, 50 μm (A–L).
