sox4b knockdown leads to decreased expression of gata2a. Fertilized eggs were microinjected with sox4bMO and analyzed by WISH for expression of lim3 and pit1 or by single fluorescent whole-mount in situ hybridization for gata2a (red) in the embryos at 48 hpf. A–C, Control; D–F, sox4bMO-injected embryos; A and D, ventral view (anterior to the top) (scale bar, 100 μm); B and E, ventral view (anterior to the top) (scale bar, 50 μm); C and F, z-plane confocal image lateral view (anterior to the left) (scale bar, 60 μm). Arrowheads in C and F point at the position of gata2a expression in the pituitary.