Fig. 9.

Both Sox4b and Gata2a are required for gonadotropin expression. Expression of lhβ and fshβ was measured by qRT-PCR in RNA extracted from sox4b morphant (A) and gata2a morphant (B) larvae of 4 dpf. The data were normalized relative to the ef1α transcript, and the levels in coMO-injected embryos were arbitrarily set to 1. Mean fold change relative to control morphants is shown with the corresponding sd (n = 3). ***, Significant at P < 0,001. The results shown are representative of three independent experiments. C, Analysis by double-fluorescent WISH for expression of lhβ and ins at 4 dpf previously microinjected with coMO or sox4bMO; pancreatic ins (arrowhead) expression was assessed as invariant control. C, Ventral view (anterior to the left). Scale bar, 200 μm.