Table 2.

Sox4b regulates expression of tshβ, gsuα, and lhβ in zebrafish

	NI normal	coMO normal	sox4bMO absence	sox4bMO decrease
Embryo, 48 hpf
tshβ	10/10	89/100	12/122	110/122a
gsuα	10/10	38/40	0/38	38/38a
glucagon	10/10	70/78	0/78	78/78a
slβ	8/10	38/40	5/40	0/40
pomc	10/10	40/40	0/40	0/40
lim3	10/10	38/38	2/40	0/40
pit1	10/10	27/27	0/35	0/35
gata2a	10/10	40/40	38/40a	0/40
gh	9/10	45/45	0/58	48/58a
prl	10/10	50/50	0/50	0/50
Larvae, 4 dpf
lhβ	10/10	50/50	0/50	28/50
tshβ	10/10	50/50	12/50a	15/50a
gsuα	10/10	50/50	0/50	30/50a
gh	10/10	50/50	0/50	25/50a
prl	10/10	50/50	0/50	0/50

Individual embryos, previously noninjected (NI) or injected with coMO or sox4bMO, were optically analyzed and compared for expression of different marker genes at 48 hpf and 4 dpf. The embryos were classified according to phenotype, and the number of each class is given relative to the total number of analyzed embryos for each condition. Absence indicates lost expression; decrease indicates that marker expression was reduced by more than 50%.

^a Significant at P < 0.001.