Fig. 4.
Tsc22d3-2-deficient BMDM are not susceptible to the immunosuppressive effects of dexamethasone. Control and knockout (A–C) and splenocytes (D and E) are incubated with or without increasing concentrations of dexamethasone (DEX, 10−10–10−7 m) and indicated stimuli. (A–C) IL-2 concentrations collected 4 h (TNF) or 18 h (IL-6, IL-12p40, and IL-2) after stimulation. Cytokine concentrations in cells stimulated without dexamethasone are set at 1 for normalization. Data are means ± sd of triplicate samples from one experiment comprising two mice per experimental condition and are representative of two experiments. ctrl, Control; ko, knockout.