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. 2012 May 8;26(7):1102–1116. doi: 10.1210/me.2011-1367

Fig. 3.

Fig. 3.

Effects of AR0Q on cell viability and neurite elongation. A, At 48 h after exposure with doxycycline or vehicle, NSC34/AR0Q were treated with 10 nm testosterone or vehicle for 120 h. *, P < 0.001 (ANOVA test). B, Effect of AR0Q expression at 24 h after testosterone treatment (10 nm) on percentage of neurite-bearing cells in the presence of serum (5%). *, P < 0.01 (ANOVA test); *, significant difference compared with I+T NSC34/AR20Q at P < 0.05 (ANOVA test). C–E, After 48 h after exposure to doxycycline (I), NSC34/AR(n)Q (n = 0 or 20) were depleted in serum and treated with 10 nm testosterone (+T) for 24 h. C and D, Percentage of neurite-bearing cells (C) and the percentage of cells with the longest neurite having a length greater than 60 μm (D). *, P < 0.05, ANOVA test. E, Immunofluorescence of serum-depleted I+T NSC34/AR0Q was performed using the peripherin antibody detected with Alexa 488-conjugated secondary antibody. Nuclei are stained with Hoechst. Peripherin, green; nuclei, blue; red scale bar, 20 μm.