Table 1.

Primers used for PCR

Gene	Identification	Primer sequence, 5′-3′	Annealing Tm. (° C)	Size (bp)
Cx26	Sense	CGGAAGTTCATGAAGGGAGAGAT	55	365
	Antisense	ACGAGTCCTTTCAGGTTTTCTGG
Cx30.3	Sense	TCAAACATGGGCCCAATG	50	182
	Antisense	CGAACGAGACACTGAGGG
Cx31	Sense	AGAAGCACGGGGAGCAAT	55	182
	Antisense	GTCACGCGGTCGTATCAT
Cx32	Sense	CTGCTCTACCCCGGCTATGC	60	386
	Antisense	TTCTCGCTGGCTACGAGTCGGAC
Cx37	Sense	GGCTGGACCATGGAGCCGGT	60	422
	Antisense	CGGGGGGTCCCACCGGCTTT
Cx40	Sense	CTGTCCCCACCCAGTCAACT	56	460
	Antisense	CGATGGTATCACTGTTTGCC
Cx43	Sense	TACCACGCCACCACTGGCCCA	57	294
	Antisense	ATTCTGGTTGTCGTCGGGGAAATC
Cx45	Sense	TTCCAAGTCCACCCATTTTAT	55	444
	Antisense	AGTCTTACCGAGTCCTTGCTA
Cx46	Sense	GGAAAGGCCACAGGGTTTCCTGG	60	332
	Antisense	GGCAACCAGGAGGACCTGGG
Cx47	Sense	TAGCCCCACAGTATGCCCTTAG	64	530
	Antisense	CGTCTGCGCTCCTGTTCC
Nanog	Sense	CACCCACCCATGCTAGTCTT	54	1071
	Antisense	ACCCTCAAACTCCTG GTCCT
Oct4	Sense	CGTGAGACTTTGCAGCCTGA	50	519
	Antisense	GGGATGTAAGTGATCTGCTG
FoxD3	Sense	TCTCTGGGGCAATCACACTC	50	550
	Antisense	GTACATTTGTTGATAAAGGG
Sox2	Sense	GGCAGCTACAGCATGATGCAGGAGC	50	171
	Antisense	CTGGTCATGGAGTTGTACTGCAGG
β-Actin	Sense	AGCCATGTACGTAGCCATCC	55	350
	Antisense	CTCTCAGCTGTGGTGGTGAA

FoxD3, Forkhead box D3; Oct4, octamer-binding transcription factor 4; Sox2, sex determining region Y box 2.