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Spatiotemporal expression of Nell-1 and Runx2 in the femurs of mouse embryos during chondrogenesis. Nell-1 and Runx2 expression in the femoral bone of WT (A) and Runx2−/− (B) embryos at embryonic day (E)14.5, E16.5, E18.5, and neonatal stage as examined by immunohistochemical staining. Boxed areas are shown below at higher magnification. Scale bars = 100 μm (A and B).
