Table 1.
Differential Expression of Select Biomarker Genes Distinguishing TDFSM from TDECC Cholangiocarcinoma Cells
| Gene name | Gene symbol | NL∗ | BDEspT† | TDECC | TDFSM | Fold change‡ | q Value |
|---|---|---|---|---|---|---|---|
| Collagen, type I, α 2 | Col1a2 | 6.88 ± 0.63 | 12.63 ± 0.40 | 3.15 ± 0.13 | 14.25 ± 0.02 | 2200.5 | 1.73×10-5 |
| Periostin, osteoblast specific factor | Postn | 4.04 ± 0.17 | 12.66 ± 0.57 | 3.18 ± 0.24 | 13.59 ± 0.25 | 1358.7 | 4.48×10-5 |
| Protease, serine, 23 | Prss23 | 7.97 ± 0.13 | 8.71 ± 0.20 | 3.69 ± 0.28 | 13.46 ± 0.10 | 875.4 | 3.93×10-5 |
| Fibronectin 1 | Fn1 | 13.50 ± 0.04 | 12.69 ± 0.23 | 4.01 ± 0.47 | 13.68 ± 0.08 | 814.3 | 8.47×10-5 |
| Serine (or cysteine) peptidase inhibitor, clade H, member 1 | Serpinh1 | 8.39 ± 0.14 | 11.33 ± 0.10 | 4.25 ± 0.24 | 13.52 ± 0.10 | 618.0 | 3.25×10-5 |
| Collagen, type I, α 1 | Col1a1 | 6.05 ± 0.33 | 12.80 ± 0.19 | 4.74 ± 0.49 | 13.83 ± 0.10 | 541.3 | 1.06×10-4 |
| Gremlin 1 | Grem1 | 3.75 ± 0.08 | 6.25 ± 1.82 | 3.34 ± 0.26 | 12.25 ± 0.11 | 482.1 | 4.33×10-5 |
| Tenascin C | Tnc | 3.81 ± 0.21 | 9.95 ± 0.64 | 3.38 ± 0.47 | 12.09 ± 0.17 | 419.6 | 1.12×10-4 |
| Insulin-like growth factor binding protein 7 | Igfbp7 | 10.90 ± 0.17 | 11.98 ± 0.21 | 4.07 ± 0.30 | 12.66 ± 0.07 | 384.6 | 4.62×10-5 |
| Secreted acidic cysteine-rich glycoprotein | Sparc | 8.11 ± 0.11 | 11.02 ± 0.12 | 4.53 ± 0.41 | 12.47 ± 0.06 | 245.3 | 9.03×10-5 |
| Chemokine (C-X-C motif) ligand 12 | Cxcl12 | 11.72 ± 0.16 | 8.28 ± 1.33 | 3.82 ± 0.08 | 11.55 ± 0.06 | 212.0 | 1.73×10-5 |
| Thymus cell antigen 1, θ | Thy1 | 5.06 ± 0.09 | 7.86 ± 0.24 | 4.18 ± 0.26 | 11.75 ± 0.11 | 189.8 | 4.83×10-5 |
| Vimentin | Vim | 8.91 ± 0.30 | 12.63 ± 0.06 | 7.94 ± 0.54 | 14.75 ± 0.01 | 112.1 | 2.05×10-4 |
| Smooth muscle α-actin | Acta2 (α-SMA) | 7.40 ± 0.64 | 10.67 ± 0.39 | 6.61 ± 0.95 | 11.60 ± 0.07 | 31.8 | 1.54×10-3 |
| Matrix metallopeptidase 2 | Mmp2 | 6.42 ± 0.21 | 10.17 ± 0.51 | 4.57 ± 0.18 | 9.31 ± 0.02 | 26.7 | 5.39×10-5 |
| Fibulin 2 | Fbln2 | 6.18 ± 0.24 | 7.64 ± 0.63 | 6.05 ± 0.27 | 10.07 ± 0.10 | 16.2 | 1.63×10-4 |
| Cofilin 1 | Cfl1 | 7.57 ± 0.22 | 8.75 ± 0.17 | 8.65 ± 0.06 | 11.17 ± 0.18 | 5.7 | 1.72×10-4 |
| Collagen, type XV, α 1 | Col15a1 | 7.14 ± 0.22 | 10.34 ± 0.83 | 6.90 ± 0.22 | 8.63 ± 0.05 | 3.3 | 6.07×10-4 |
| Desmin | Des | 6.71 ± 0.04 | 8.11 ± 0.40 | 5.72 ± 0.09 | 6.35 ± 0.05 | 1.5 | 1.06×10-3 |
| Oncomodulin | Ocm | 4.39 ± 0.28 | 12.95 ± 0.17 | 13.12 ± 0.22 | 9.28 ± 0.10 | −14.4 | 1.35×10-4 |
| Mucin 1, transmembrane | Muc1 | 5.96 ± 0.25 | 9.55 ± 0.95 | 9.62 ± 0.95 | 5.42 ± 0.11 | −18.4 | 2.30×10-3 |
| Mesothelin | Msln | 6.19 ± 0.26 | 11.22 ± 0.53 | 12.84 ± 0.31 | 5.83 ± 0.17 | −129.2 | 8.56×10-5 |
| Tumor-associated calcium signal transducer 1 (Epcam) | Tacstd1 | 6.96 ± 0.27 | 13.43 ± 0.30 | 12.88 ± 0.31 | 3.20 ± 0.12 | −821.4 | 4.50×10-5 |
| CD24 antigen | Cd24 | 5.08 ± 0.07 | 12.28 ± 0.53 | 12.56 ± 0.60 | 3.58 ± 0.03 | −503.5 | 1.47×10-4 |
| Cadherin 1 | Cdh1 | 10.00 ± 0.33 | 12.08 ± 0.34 | 12.77 ± 0.35 | 3.48 ± 0.12 | −623.2 | 5.55×10-5 |
| Keratin 19 | Krt19 | 5.45 ± 0.22 | 13.88 ± 0.36 | 14.34 ± 0.19 | 3.58 ± 0.18 | −1731.6 | 2.71×10-5 |
Data are expressed as means ± SEM unless otherwise indicated. n = 3 samples analyzed per individual specimen group.
TDECC, cholangiocarcinoma cells; TDFSM, cancer-associated myofibroblasts.
∗
Normal liver (NL) is represented by cholangiocarcinoma-free right liver lobe tissue pair-matched with BDEspT from the same animal.
†
BDEspT, orthotopic rat cholangiocarcinoma from which TDFSM and TDECC cell strains were derived.
‡
Fold change of gene expression in TDFSM versus TDECC.