Fig. 6.

DEX recruits HDAC1 to the Runx2 P2 promoter. A, Postconfluent 3T3–L1 preadipocytes were induced to differentiation as described in the text. ChIP-quantitative PCR was performed from d 0 to d 6 after induction with antiacetylhistone H4 antibody and nonspecific IgG. B, 3T3–L1 preadipocytes were treated with inducing medium with different combination of the MDI inducers, and ChIP-quantitative PCR was performed at 24 h after induction with antiacetylhistone H4 antibody, nonspecific IgG being used as negative control. C, Postconfluent 3T3–L1 preadipocytes were induced with MI or MDI, and ChIP-quantitative PCR was performed at 24 h after induction with anti-HDAC1 or anti-HDAC2 antibodies, nonspecific IgG being used as the negative control. D, 3T3–L1 preadipocytes were transfected with HDAC1 siRNA, and 48 h after reaching confluence, cells were induced to differentiate as described in the text. Cells were treated with MI or MDI for 24 h, and Runx2 mRNA levels were measured. The knockdown effect was detected by Western blotting. E–G, 3T3–L1 preadipocytes were transfected with GR siRNA, and 48 h after reaching confluence, cells were induced with MI or MDI for 24 h. ChIP-quantitative PCR was performed at 24 h after induction with anti-GR, anti-HDAC1, antiacetylhistone H4 antibodies and nonspecific IgG. Rel, Relative.