Fig. 1.
Role of ERα palmitoylation on E2-induced receptor degradation in MCF-7 cells. Panels A and B, [3H]Palmitate incorporation (A) and ERα immunofluorescence staining (B) in MCF-7 cells treated with the PAT inhibitor 2-Br (10 μm, 30 min); panel C, Western blot analysis of ERα cellular levels in MCF-7 cells treated with E2 (10 nm) at different time points in the presence of 30 min pretreatment with the PAT inhibitor 2-Br (10 μm) before E2 administration. Inhibitor alone was administrated for 2 h 30 min. Loading control was done by evaluating vinculin expression in the same filter. *, Significant differences with respect to the relative control sample; °, significant differences with respect to the corresponding E2 sample (P < 0.05). Representative blots are shown. Arrows indicate membrane ERα. C, Control.