Fig. 6.

Impact of CtBP1 knockdown and LPA treatment on CtBP1-mediated hypoxic apoptosis. Pooled clones (n = 3) of GH4 pituitary cells with scrambled control (A) or shRNA targeting CtBP1 (D) were examined by flow cytometry after Annexin V staining. The proportion of cells undergoing early apoptosis is shown in the Q3 window (red circles). To examine the impact of hypoxia, cells were also examined under 0.2% O₂ conditions. CtBP1 knockdown cells (E) show greater susceptibility to hypoxia-induced apoptosis compared with controls (B). Concomitant treatment with LPA 5 μm (C and F) rescues hypoxia-induced apoptosis in CtBP1 knockdown cells. Data shown are representative of two independent experiments.