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Stable knockdown of Enpp2 diminishes GH4 cell growth and hormone expression. A, Pooled clones (n = 3) of GH4 pituitary cells with scrambled shRNA (control) or shRNA targeting Enpp2 were examined by Western blotting. B, Cell counting demonstrates reduced cell growth rate of Enpp2 knockdown cells compared with controls. Data shown are representative of three independent experiments.
