Fig. 2.
Delayed postnatal differentiation of OPC after ubiquitous TRα1L400R expression. A, Quantification of mRNA relative abundance in whole cerebellum at different ages (P8, P15, and P21). Comparison between ubiquitous mutants (TRαAMI/S) mice (n = 6, in black) and control littermates (n = 6, in gray). PDGFRα is specific of OPC, and Mbp is a marker of mature OL. P15 wild-type whole cerebellum RNA was used as a reference. B, Myelin basic protein quantification at P15 by Western blot analysis (n = 5), normalized by β-tubulin (β-Tub) indicates a delay in myelination in mutant expressing the TRα1L400R ubiquitously (TRαAMI/S). Data are expressed ± sd. *, Significant difference between mutants and wild types, P < 0.05. MBP, Myelin basic protein.