Table 3.
Quantitative RT-PCR analysis of gene expression in whole cerebellum at P8 and P60
| A. Total cerebellar mRNA at P8 | |||
|---|---|---|---|
| Gene | Control group mean (±sd) (n = 6) | TRαAMI/N mean (±sd) (n = 6) | TRαAMI/P mean (±sd) (n = 6) |
| Ntf-3 | 100 ± 6.2 | 67 ± 6.6a | 144.0 ± 14.9a |
| Ngf | 100 ± 28.2 | 99.3 ± 12.3 | 96.6 ± 11.1 |
| Bdnf | 100 ± 49.6 | 20 ± 2.4a | 152.0 ± 54 |
| Pdgf | 100 ± 5.4 | 79 ± 8.4a | 101.9 ± 22.1 |
| p75 | 100 ± 6.5 | 37 ± 4.4a | 89.3 ± 5.1 |
| Shh | 100 ± 13.2 | 46 ± 4.7a | 105.4 ± 35 |
| Gli2 | 100 ± 8.9 | 26 ± 3.4a | 126.8 ± 39.7 |
| Ptch1 | 100 ± 18.1 | 33 ± 5.0a | 114.8 ± 16.5 |
| Gabra6 | 100 ± 16.5 | 42 ± 18.3 | 109 ± 8.7 |
| B. Total cerebellar mRNA at P60 | |||
|---|---|---|---|
| Gene | Control group cre-mean (±sd) (n = 5) | TRαAMI/Pt2/P30 mean (±sd) (n = 5) | TRαAMI /Pt2 no tamoxifen mean (±sd) (n = 5) |
| Olig2 | 100 ± 25.3 | 198.0 ± 34.2a | 93.0 ± 16.2 |
| Pdgf-aa | 100 ± 14.2 | 102.3 ± 34.5 | 107.8 ± 20.1 |
| Plp | 100 ± 16.5 | 92.5 ± 43.9 | 128.0 ± 17.1 |
| Mbp | 100 ± 16.3 | 76.1 ± 15.3 | 97.8 ± 19.0 |
| Golli Mbp | 100 ± 24.0 | 78.3 ± 14.7 | 108.2 ± 25.3 |
| TRa1 | 100 ± 20.8 | 135.0 ± 25.6 | 106.3 ± 12.5 |
Data normalized using Hprt expression as a reference.
a
Significant difference (bold characters), P < 0.05.