Fig. 3.
ERβ, AR, and PELP1 can form a complex on DNA. A, Fifty micrograms of protein extracts from PC-3 cells or PC-3(AR)2 (PC-3 cells stably transfected with AR under the control of its native promoter) were evaluated by immunoprecipitation by AR, ERβ, and control and immunoblotted with corresponding antibody. Input lysates are shown on the left. B, One hundred microliters of sheared DNA lysates from PC-3 cells or PC-3-AR2 were subject to ChIP assays with the PSA promoter after immunoprecipitation with ERβ, AR, PELP1, or IgG control. A representative PCR gel is shown below. IB, Immunoblot; IP, immunoprecipitation; *, statistically significant.