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. 2011 Dec 1;26(1):67–78. doi: 10.1210/me.2011-1090

Fig. 6.

Fig. 6.

Cadherin-16 expression is modulated by TSH in FRTL-5 thyroid cells. A, FRTL-5 cells were cultured in regular medium (C) or maintained in starvation medium for 7 d (T0) and then treated with 1 mU/ml of TSH for different times (12, 24, and 48 h) and analyzed by qRT-PCR analysis to measure the expression of Pax8 (black bars) and Cadherin-16 (CDH16) (gray bars) mRNA. The values are means ± sd of three independent experiments in duplicate, normalized by the expression of β-actin, and expressed as fold change with respect to the control, whose value was set at 1.0. Statistical analysis uses t test (P < 0.01). B, FRTL-5 cells were cultured in regular medium (lane C) or maintained in starvation medium for 7 d (lane 0) and then treated with 1 mU/ml TSH for different times (12, 24, and 48 h) and analyzed by Western blotting to detect Pax8 and Cadherin-16 proteins. The hybridization with α-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) assessed the protein uniform loading and integrity.