Fig. 4.
Effect of I− treatment on intracellular ROS levels. Measurements were done by DCFDA method after 6, 24, 48, and 72 h I− treatment. PCCl3 cells treated with 5 μm H2O2 for 6 h (gray bar) were used as assay control. Data represent the mean ± se of at least three independent experiments performed in triplicate. Statistical significance was evaluated by by Student's paired t test. Differences vs. control (c, black bars) for each time point normalized to 1 were considered significant: *, P < 0.05; **, P < 0.01; ***, P < 0.001.