Table 1.
Gene expression analyses in WT, Igf1r+/− and Igf1r−/− thyroids of one year old male and female mice
|
Tg |
Nis |
Tpo |
Igf2r |
Tshr |
Insr |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | ±sd | n | Mean | ±sd | n | Mean | ±sd | n | Mean | ±sd | n | Mean | ±sd | n | Mean | ±sd | n | |
| Igf1r +/+, m | 1.00 | 0.32 | 4 | 1.00 | 0.28 | 5 | 1.00 | 0.22 | 4 | 1.00 | 0.15 | 5 | 1.00 | 0.31 | 5 | 1.00 | 0.20 | 5 |
| Igf1r +/−, m | 0.81 | 0.48 | 5 | 1.07 | 0.30 | 5 | 0.65 | 0.07 | 5 | 1.28 | 0.20 | 5 | 1.75 | 0.24 | 5 | 0.75 | 0.14 | 5 |
| Igf1r −/−, m | 0.74 | 0.33 | 5 | 1.41 | 0.13 | 5 | 0.80 | 0.10 | 5 | 1.78 a | 0.14 | 5 | 1.97 | 0.84 | 5 | 1.19 | 0.12 | 5 |
| Igf1r +/+, f | 1.00 | 0.44 | 4 | 1.00 | 0.25 | 7 | 1.00 | 0.07 | 5 | 1.00 | 0.08 | 5 | 1.00 | 0.08 | 5 | 1.00 | 0.06 | 5 |
| Igf1r +/−, f | 1.36 | 0.72 | 5 | 0.83 | 0.12 | 5 | 0.42a | 0.08 | 5 | 1.93b | 0.43 | 5 | 1.20 | 0.26 | 5 | 1.03 | 0.27 | 5 |
| Igf1r −/−, f | 1.02 | 0.29 | 4 | 0.64 | 0.27 | 6 | 0.33c | 0.05 | 4 | 1.58c | 0.18 | 4 | 0.96 | 0.38 | 4 | 1.00 | 0.13 | 4 |
Analyzed are the Tg, Nis, Tpo, Igf2r, Tshr, and Insr mRNA expression levels with Taqman probe kits from Applied Biosystems. Shown are the means of 2ddCT values of the respective gene (no bold) normalized to the housekeeping gene Hmbs compared to the WT mice. Student's t test was performed per genotype and sex (P < 0.05 was considered significant).
a
P < 0.01;
b
P < 0.01;
c
P < 0.001. f, Female; m, male.