Table 4.
Mutations in rdxA and frxA that were associated with metronidazole resistance
| No. | Strains | MIC (mg/L) | Profile | rdxA mutations | frxA mutations |
|---|---|---|---|---|---|
| 1 | DM5 | 2 | Sensitive | None | None |
| 2 | DM20 | 1 | Sensitive | None | None |
| 3 | DM63 | 4 | Sensitive | P44T, K168E, W209C | V34A |
| 4 | DM92 | 2 | Sensitive | E194Q | Poor sequence quality |
| 5 | DM95 | 1 | Sensitive | H25R | None |
| 6 | DM4 | 64 | Resistant | None | Q27E, N111H, N129T, S130D |
| 7 | DM7 | 64 | Resistant | A68T, V85A, 150 frameshift | Poor sequence quality |
| 8 | DM8 | 128 | Resistant | R16C, S108A, D205A | A70V |
| 9 | DM10 | 64 | Resistant | S43W | Q27E, A70G, A85V, N111H, N129T, S130D, E176K, N182D |
| 10 | DM14 | 64 | Resistant | R10K, P44L, K168R, K198I, R200* | Q27E, M126I, N129T, S130D, A153V, E176K, N182D |
| 11 | DM32 | 64 | Resistant | G170S | G45R |
| 12 | DM39 | 64 | Resistant | K198I, K201E | Q27E, N111H, N129T, S130D, E176K |
| 13 | DM43 | 32 | Resistant | 41frameshift | Q27E, N111H, N129T, S130D, N182D |
| 14 | DM44 | 64 | Resistant | E75* | Q27E, N111H, N129T, S130D, E176K |
| 15 | DM50 | 64 | Resistant | Q50* | Q27E, A32E, N111H, N129T, S130D, E176K |
| 16 | DM51 | 32 | Resistant | G145R, E174G, | A70V |
| 17 | DM52 | 64 | Resistant | 120frameshift | Q27E, A70G, N111H, N129H, S130D, E169A, E176K |
| 18 | DM57 | 64 | Resistant | 3frameshift | Q27E, A70G, 86frameshift |
| 19 | DM60 | 64 | Resistant | R16C, P96L | None |
| 20 | DM62 | 64 | Resistant | A68T, G189S | Q27E, P41L, A70G, N111H, N129T, S130D, M146I |
| 21 | DM64 | 64 | Resistant | 9frameshift | Q27E, A32E, N111H, N129T, S130D, E176K, N182D |
| 22 | DM73 | 64 | Resistant | L188F, K190* | None |
| 23 | DM75 | 64 | Resistant | K190Y, A193* | Q27E, A32E, T110A, N111H, N129T, S130D, E176K |
| 24 | DM76 | 128 | Resistant | 122–149del, D205A | Poor sequence quality |
| 25 | DM79 | 64 | Resistant | S30G, E38* | Q27E, N111H, N129T, S130D, E176K |
| 26 | DM81 | 128 | Resistant | 42frameshift | V103I |
| 27 | DM84 | 64 | Resistant | G122S | Q27E, A32E, N111H, N129T, S130D, E176K, N182D |
| 28 | DM86 | 64 | Resistant | T31H, V57A, L62V, K64N, P106S, G155* | None |
| 29 | DM87 | 64 | Resistant | V85G, G145E, V151A, V192Q, A193G, E194R, Q197E, R200* | Q27E, N111H, N129T, S130D, E176K |
| 30 | DM88 | 32 | Resistant | R16H, R200M, S202I, 204frameshift | None |
| 31 | DM89 | 64 | Resistant | 65frameshift | Q27E, N111H, N129T, S130D, E176K, N182D |
| 32 | DM96 | 64 | Resistant | E35* | Q27E, N111H, M126I, N129T, S130D, E176K, N182D |
| 33 | DM100 | 32 | Resistant | S108A, K190E | A153V, E176K |
| 34 | DM103 | 32 | Resistant | C19* | Poor sequence quality |
| 35 | DM104 | 64 | Resistant | R16H, 207del | 22frameshift |
| 36 | DM105 | 16 | Resistant | P51S, K203Q | 18frameshift |
| 37 | DM112 | 64 | Resistant | 27frameshift | Q27E, N111H, N129T, S130D, E176K, N182D |
| 38 | DM115 | 64 | Resistant | R16H, H97Y, V182A, R200* | A85V, N111H |
| 39 | DM117 | 32 | Resistant | H69P, V85G, V86E, Q139H, L153* | Q27E, L72I, N111H, M126I, N129T, S130D, E169A, E176K, N182D |
| 40 | DM118 | 32 | Resistant | S18F, S43L, K63Q, G155R, K198T, R200* | Q27E, N111H, M126I, N129T, S130D, E176K, N182D |
| 41 | DM126 | 64 | Resistant | V85G, F117V, G122A, I147L, K198I, 208frameshift | Q27E, N111H, N129T, S130D, E169A, N182D |
| 42 | DM142 | 64 | Resistant | R16H, G122A, P180T, 197frameshift | Q27E, N111H, N129T, S130D, N182D |
| 43 | DM143 | 64 | Resistant | Q50* | 18frameshift |
| 44 | DM146 | 64 | Resistant | T31H, 192frameshift | Poor sequence quality |
| 45 | DM147 | 64 | Resistant | R16Y, H25Y, E35D, L62F, Q146H, R200* | 18frameshift |
| 46 | DM148 | 128 | Resistant | R16C, R166S, 198frameshift | A153V |
| 47 | DM150 | 16 | Resistant | Q197H, 200frameshift | R38I |
| 48 | DM151 | 128 | Resistant | C140Y, Q197* | N111H, N129T, S130D, E176K |
| 49 | DM152 | 64 | Resistant | E35* | Q27E, N111H, N129T, S130D, N182D |
MIC = minimum inhibitory concentration. None: no specific mutations were identified; A68T means threonine replaced alanine in amino acid position 68; R200* means a stop codon replaced arginine in amino acid position 200; 150frameshift means there was a frameshift mutation in amino acid position 150. We ignored mutations that were present in both sensitive and resistant strains.