Table 5.

Mutations in other genes that were associated with metronidazole resistance

No.	Strains	MIC (mg/L)	Profile	rpsU (hp0562)	rps4 (hp1294)	dppA (hp0298)	dppB (hp0299)	rnc (hp0662)	dapF (hp0566)	recA (hp0153)	fdxA (hp0277)	fdxB (hp1508)	trx1 (hp0824)
1	DM30	1	Sensitive	None	None	35del, 36del, 37del, 179H	None	None	None	None	None	None	E9G
2	DM116	1	Sensitive	None	None	None	None	None	None	None	None	None	None
3	DM76	128	Resistant	None	A48T, V170A	E473G	I322V	P15L	V16A, A84N, I109V, K117E, E161G, G162L, R221C, G248E, A257V, R266G, E269K	None	N32S, S79I	V64I, M65L, P245A, I304T, A428I, A439T	S14N
4	DM151	128	Resistant	A34T	None	T32A, E473G	R124C	1del, N152K,	K50N, N164F, E177A, R221C, G248E, A257I, R266G, G271H	None	43del, 48del, S79I	F125L, A227V, N231K, P245A, R307H, M321L, A325T	S14N
5	DM4	64	Resistant	None	None	None	K199R, I322V	1del	V16A, K50R, D51N, A84N, K117E, E161G, G162L, G248E, A245V, R266G, G271H	None	N32S, S79I	A38V, V64I, M65L, A227V	None
6	DM64	64	Resistant	C32Y	None	E473G	I322V	P15S	V16A, K50R, D51N, A84N, I109V, K117E, E161G, G162L, G248E, A257V, R266G, E269K	None	N32S, S79I	V64I, M65L, P245A, H311A, N395S, N424H	None
7	DM18	32	Resistant	None	None	E473G	I21V, V127M, I322V	I13A, R109H	V16A, A84N, I109V, K117E, E161G, G162L, G248D, A257V, R266G, E269K	None	N32S, S79I	V64I, M65L, M163V, P245T, S417G, A428I	N102S
8	DM51	32	Resistant	None	None	Q383E	None	None	K129N, A254T, V267I	S40D, I191V	N32S, S79I	S122R, P123S, D170E, I173V, P245A, N424S	I40V

MIC = minimum inhibitory concentration; None: no specific mutations were identified; 35del means there was a deletion at amino acid position 35; I79H means histidine replaced isoleucine in amino acid position 79. We ignored mutations that were present in both sensitive and resistant strains.