Fig. 1.

ERα interacts with CrkL in vivo. A, Immunoprecipitation with ERα antibody (sc-542) using the nuclear extract of the periimplantation period. Twelve bands were obtained marked GB1–GB12. GB5 and GB9 correspond to ERα and CrkL, respectively. B, Western blot analysis with ERα immunoprecipitate using ERα antibody (sc-542) to prove the presence of ERα in the IP. C, ERα IP blot probed with CrkL (sc-319). D, Western blot analysis of ERα on ERα immunoprecipitate of different days of pregnancy using the ERα antibody and CrkL antibody. See also Supplemental Fig. 1. E, Mammalian two-hybrid assay of HEK cells transfected with pACT ERα and pBIND CrkL before and after estrogen treatment. The effect of estrogen and vectors (pACT ERα alone, pBIND CrkL alone, and pACT+ pBIND + G5Luc) on basal reporter activity is also shown. IP, Immunoprecipitate; WB, Western blot; GB, gel band.