Fig. 2.

Mutant MC4R forms intracellular inclusions in a neuronal cell line. A and B, Confocal analyses of HEK293 (A) or SK-N-SH (B) cells expressing either WT MC4R-GFP, MC4R(S58C)-GFP, MC4R(P78L)-GFP, or MC4R(D90N)-GFP. Scale bar, 10 μm. C, Quantification of the incidence of inclusions in WT and mutant MC4R-GFP expressing cells. A minimum of 50 transfected cells was scored for each treatment from three separate transfections. Scoring was conducted blind to experimental status 24 h after transfection. Error bars represent 2× se. *, P < 0.05.