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Functional expression of MC4R mutants is increased in cells with increased levels of Hsc70. MC4R signaling in cells stimulated with 10−6 m NDP-MSH for 6 h was quantified by a dual luciferase reporter assay for cAMP activity. Cells with transient expression of WT or mutant HA-MC4R were cotransfected with vectors for expression of V5-Hsc70 or empty vector and luciferase reporter vectors. Error bars represent 2× se. *, P < 0.05.
