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. 2011 Aug 4;25(9):1550–1564. doi: 10.1210/me.2011-0106

Fig. 3.

Fig. 3.

Identification of a novel PRLr TAD. A, Top, Schematic of Gal4-UAS luciferase (Luc) reporter and Gal4-DBD PRLr fusion construct; bottom, schematic of Gal4-ECD, Gal4-ICD, Gal4-Stat5aTAD, or Gal4 control constructs. B, Gal4-UAS luciferase activity in 293T cells transfected with Gal4-ECD, Gal4-ICD, Gal4-Stat5aTAD, or Gal4 control constructs. C, Gal4-UAS luciferase assay measuring transactivation of PRLr C-terminal truncation constructs in 293T cells. D, Gal4-UAS luciferase activity of Gal4 fusion constructs in 293T cells with the indicated PRLr regions as determined by C. E, Cross-species sequence alignment of the identified PRLr TAD highlighting four conserved residues (gray). F, Gal4-UAS luciferase activity of the TAD point mutations in 293T cells as determined in F. Reported values are those of a representative experiment repeated three separate times, where sem indicates the error of quadruplicate transfections performed within one experiment. All P values were calculated using one-way ANOVA with Bonferroni multiple-comparisons test. RLU, Relative light units. **, P < 0.01; ***, P < 0.001.