Fig. 6.

HMGN2 knockdown impairs PRLr and Stat5a promoter binding. A and B, shNS or shHMGN2 cells were serum starved for 24 h before PRL treatment. Cells were then subjected to ChIP using anti-PRLr (A) or anti-Stat5a (B) antibodies. Results were normalized to ChIP results obtained using an IgG control antibody in untreated shNS cells; values are reported as fold change. The values are those of a representative experiment repeated two separate times, where sem indicates the error of three replicate measurements within one single experiment. All P values were calculated using one-way ANOVA with Bonferroni multiple-comparisons test. Calculated P values of note are highlighted in the figure. shNS represents T47D cells expressing control shRNA; shHMGN2 represents T47D cells expressing shRNA against HMGN2. *, P < 0.01; **, P < 0.01; ***, P < 0.001.