Fig. 7.

The PRLr TAD promotes a transformed phenotype. A, MCF10AΔp53 transfectants were suspended in a 0.3% agar-medium mix supplemented with PRL (250 ng/ml) for 10 d. Each condition was photographed under phase-contrast optics. B, Quantification of the average colonies per field. Each condition was set up in triplicate wells, and error bars represent sem. All P values were calculated using one-way ANOVA with Bonferroni multiple-comparisons test. Calculated P values of note are highlighted in the figure. PRLrYDmut is transactivation-deficient PRLr harboring Y406F/D411A mutations. ***, P < 0.001.