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Functional characterization of zebrafish TSHR. Intracellular cAMP production was assessed in HEK293T cells transiently transfected with zebrafish tshr/pcDNA3.1 expression vector after stimulation with bovine TSH (bTSH), recombinant human TSH (rhTSH), and hCG. cAMP generation was measured by homogeneous time-resolved fluorescence. Data are expressed as means of triplicate measurements.
