. 2011 Jul 7;25(9):1579–1599. doi: 10.1210/me.2011-0046

Table 1.

Quantitative analysis of the number of embryos and larvae showing reduced gene expression resulting from microinjection of tshr-MO

Molecular marker
Stage^a	PTU^b	nkx2.1a	hhex	pax2a	pax8	foxe1	tg	tpo	slc5a5	iyd	bcl2l1
55 hpf	None	37%^c	41%	28%	42%	39%	51%	44%	59%	49%	40%
		25/68^d	24/58	21/74	31/73	21/54	31/61	33/75	41/69	37/75	23/57
	0.003%	42%	39%	31%	40%	40%	48%	55%	71%	58%	36%
		33/77	24/61	20/64	22/55	24/60	30/62	34/62	52/73	38/65	24/67
100 hpf	None	100%	46%	n.q.^e	78%	n.d.^f	31%	100%	100%	100%	n.d.
		78/78	29/63		51/65		20/64	84/84	81/81	74/74
	0.003%	99%	81%	n.q.	97%	n.d.	61%	100%	100%	100%	n.d.
		70/71	48/59		59/61		46/76	78/78	76/76	72/72

Staining characteristics of thyroid tissue were compared between embryos injected with 2.5 ng tshr-MO or equimolar amounts of standard control MO. Sample size in the control group was n = 57–81 embryos per gene and time point. All analyses were performed with casper embryos. n.q., Not quantified; n.d., not detectable.

^{^a}

Developmental stage in hpf at which embryos were sampled and fixed for analyses.

^{^b}

Analyses were performed for embryos raised in the absence or presence of 0.003% PTU in embryo medium.

^{^c}

Percentage of tshr morphants displaying reduced expression of a given thyroid marker.

^{^d}

Number of tshr morphants displaying reduced expression/total number of tshr morphants analyzed.

^{^e}

Effects not quantified (n.q.) due to very low or absent staining in control larvae at 100 hpf.

^{^f}

Expression not detectable (n.d.) in thyroid of tshr morphants and control larvae at 100 hpf.