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. 2011 May 26;25(8):1364–1375. doi: 10.1210/me.2010-0503

Fig. 3.

Fig. 3.

Expression levels of SF-1 affect StarD7 promoter activity. A, JEG-3 cells were transiently cotransfected with 938StarD7Luc, 673StarD7Luc, or 312StarD7Luc and phRL-TK Renilla constructs and SF-1 expression plasmid as indicated. Relative luciferase reporter gene activity was normalized against the Renilla activity, and results were expressed as the fold increase related to the activity in cells cotransfected with the empty expression vector defined as 1-fold. The values represent the mean ± sem of triplicate experiments. B, Quantitative real-time PCR of StarD7. Total RNA was extracted from JEG-3 cells 24 h after transfection with SF-1 expression plasmid as indicated. StarD7 mRNA level was determined by real-time RT-PCR, normalized against cyclophilin A using the comparative 2−ΔΔCt method (57). Values for StarD7 mRNA represent the mean ± sem of triplicate experiments. *, P < 0.05 compared with cells cotransfected with the empty expression vector.