Fig. 4.

cAMP and SF-1 mediate StarD7 promoter response. JEG-3 cells were transiently cotransfected with 938StarD7luc, and pRL-TK Renilla constructs and SF-1 expression plasmid (200 ng). Twenty-four hours after transfection, the medium was replaced, and where indicated, the cells were treated or not with 10 μm of FSK. Relative luciferase reporter gene activity normalized against the Renilla activity was calculated, and results were expressed as the fold increase related to the activity in cells cotransfected with the empty expression vector and without FSK treatment defined as 1-fold. *, P < 0.05 compared with cells transfected with the empty expression vector and without FSK treatment; #, P < 0.05 compared with cells transfected with the SF-1 expression vector and without FSK treatment.