Fig. 7.

SF-1 transcription factor and β-catenin specifically binds to the StarD7 promoter. S33Y β-catenin and SF-1 transfected-JEG-3 cells cultured during 24 h were fixed with formaldehyde, and chromatin solutions were subjected to ChIP analyses using the following antibodies: lane 1, anti-SF-1; lane 2, anti-β-catenin; lane 3, unrelated anti-PSG antibody; and lane 4, control without antibody. The obtained DNA was analyzed by PCR using primers to amplify the StarD7 promoter region (A) or the control genomic region of iNOS (B). The results are representative of three independent experiments. IP, Immunoprecipitated samples.