Fig. 2.

ChREBP activates the MIG12 promoter. A, HEK293 cells were transfected with 200 ng of the indicated reporter constructs together with 50 ng of pCMV-β-gal in the presence or absence of 20 ng of expression plasmids for ChREBP and Mlx. Forty-eight hours after transfection, luciferase assays were performed as described in Materials and Methods. Promoter activity of pMIG12-2.5kb-Luc in the absence of ChREBP and Mlx is represented as 1. B, Pooled livers from four mice were analyzed by ChIP assays using an antibody for ChREBP and an isotype-matched IgG control antibody. Bound DNA was quantitated by real-time PCR and normalized to the input. All results are expressed as mean ± sd of three independent experiments. *, P < 0.05; **, P < 0.01; KO, knockout; IP, immunoprecipitation.