Fig. 3.

LXR and ChREBP synergistically regulate the MIG12 promoter. HEK293 cells were transfected with 200 ng of the indicated reporter constructs together with 50 ng of pCMV-β-gal in the presence or absence of 20 ng of the indicated expression plasmids for LXRα, RXRα, ChREBP, and Mlx. Twenty-four hours after transfection, cells were placed in medium A containing the vehicle (DMSO) or 10 μm T0901317. After incubation for another 24 h, luciferase assays were performed as described in Materials and Methods. Promoter activity of pMIG12-2.5kb-Luc in the absence of LXRα, RXRα, ChREBP, and Mlx with vehicle treatment is represented as 1. Values are averages from three experiments. All results are expressed as mean ± sd. KO, Knockout.