Fig. 4.

Glucose and LXR ligand synergistically regulate MIG12 gene expression in rat primary hepatocytes. Hepatocytes were cultured for 24 h in medium C (medium 199 supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 100 nm insulin, and 100 nm dexamethasone) containing 5 mm glucose. Cells were then placed in medium C containing 5 mm glucose (L) or 25 mm glucose (H) in the presence or absence of 10 μm T0901317. After incubation for another 24 h, total RNA was extracted, and real-time PCR analysis was performed. Relative mRNA levels were obtained after normalizing to 36B4 mRNA levels. mRNA levels (MIG12, ABCA1, and L-PK) in the presence of 5 mm glucose without T0901317 are represented as 1. Values are averages from three experiments. All results are expressed as mean ± sd. Different letters above the bars indicate a significant difference between groups.