MIG12 expression in livers of fasted and refed mice. Mice in the fasted group (F) (n = 5) were fasted for 24 h, and those in the refed group (R) (n = 5) were fasted for 24 h and then refed the normal diet for 24 h. Total RNA was extracted, and real-time PCR analysis was performed. Relative mRNA levels were obtained after normalizing to 36B4 mRNA levels. The mRNA levels (MIG12, PEPCK, and L-PK) under the fasted condition are represented as 1. All results are expressed as mean ± sd. **, P < 0.01.