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. 2011 Apr 7;25(6):995–1005. doi: 10.1210/me.2011-0070

Fig. 7.

Fig. 7.

Effect of MIG12 on lipogenic gene expression in rat primary hepatocytes. A, Hepatocytes were infected with 20 MOI of adenoviruses for the expression of β-gal (Ad-Con) or mMIG12 expression (Ad-MIG12) as described in Materials and Methods. At 24 and 48 h after infection, cells were placed in fresh medium B containing the vehicle (DMSO) or 10 μm T0901317. Seventy-two hours after infection, total RNA was extracted, and real-time PCR analysis was performed. Relative mRNA levels were obtained after normalizing to cyclophilin mRNA levels. mRNA levels (FAS and ACC) in cells infected with Ad-Con in the absence of T0901317 are represented as 1. B, Hepatocytes were treated and analyzed in the manner described for Fig. 6C. mRNA levels (MIG12, FAS, and ACC) of cells transfected with control siRNA (siCon) in the absence of T0901317 are represented as 1. All results are expressed as mean ± sd; experiments were performed in triplicate. Similar results were obtained in three independent experiments. *, P < 0.05; **, P < 0.01.