Fig. 2.

The effect of the LP2 dietary regime on hepatic Cyp7a1 protein levels in rat offspring at embryonic d 19 (A), male (B), and female (C) rat offspring at postnatal d 21 and male (E) and female (F) rat offspring at postnatal d 130. D, The effect of LP3 dietary regime on hepatic Cyp7a1 protein levels in male rat offspring at postnatal d 21. Relative hepatic Cyp7a1 protein levels were determined using Western blot analysis. Total membrane protein was isolated and Cyp7a1 protein was detected on a Western blot using the Cyp7a1 primary antibody. The Cyp7a1 protein level was quantified using densitometry and normalized to that of β-actin protein levels. Results were expressed as the mean ± sem. *, Significant difference (P < 0.05); n = 4–7/group, where each n represents a single offspring derived from a different mother.