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. 2011 Mar 3;25(5):785–798. doi: 10.1210/me.2010-0395

Fig. 4.

Fig. 4.

The effect of LP2 dietary regime on the in vivo level of acetylated histone H3 [K9,14] (A, C, E, and G) and trimethylated histone H3 [K9] (B, D, F, and H) at the hepatic Cyp7a1 LXRE site in rat offspring at embryonic d 19 (e19), male rat offspring at postnatal d 21, male and female rat offspring at postnatal d 130, respectively. ChIP was carried out on snap-frozen liver tissues by immunoprecipitation with antibodies for acetylated histone H3 [K9,14] and trimethylated histone H3 [K9]. Quantification analysis on the immunoprecipitated solubilzed DNA was carried out by real-time PCR via use of primers specific for the LXRE site on the promoter regions of hepatic Cyp7a1. The relative level of immunoprecipitated genomic DNA was normalized to the total genomic DNA. Results were expressed as the mean ± sem.*, Significant difference (P < 0.05); n =4–6/group, where each n represents an offspring derived from a different mother. RXR, Retinoid X receptor.