Fig. 2.

Overview of novel features and tools of the web application update. A, 2D – Snake-Plot – Search representing the requested mutation data in a 2D plot of each GPHR sequence and hyperlinking those to the detailed mutation information. The appearance can be modified interactively by the user. B, The 3D Structural Morphing between basal and activated receptor conformations permits an interactive trace of any amino acid changes, including those of the surrounding residues during a potential molecular activation event. C, The Interactive 3D Search allows picking of any particular amino acid positions of interest on a 3D structure or homology model by providing the user with information about available side-chain variations and their particular functional effects that can be mapped onto the selected 3D structure or model. D, Analysis of Multiple Mutations mapped onto 3D structures provides insight into functional effects, such as whether they result from the cooperative effect of double or triple mutations.