Fig. 3.

Additive effect of FSH and LH. KK1 cells were cotransfected with 1 μg of the −3068hAMH-luc construct and cDNAs (1 μg) of the FSH and LH receptors. Luciferase activity was assessed 48 h after stimulation by cAMP (1 mm), FSH (1 IU/ml), LH (1 IU/ml), FSH and LH (1 IU/ml of each), or HMG (1 IU/ml) (A) and combinations of FSH and LH at different ratios (0.5, 0.75, 1. 1.5, 2) in two ranges of concentrations (10–20 and 50–100 mIU/ml) (B). Results are expressed as percentage of stimulation. Comparisons of means between different treatments (A) were made by repeated-measures ANOVA, followed by Dunnett post hoc test to compare all vs. cAMP. Comparison of means between LH to FSH ratios (B) were made using the nonparametric Mann and Whitney U test. Data shown correspond to the mean ± sem of at least three experiments, each done in triplicate. NS, Not significant; **, P < 0.01; ***, P < 0.001.