Table 2.

Sequence of the primers and kits used for targeted mutagenesis

Mutations	Sequences	Targeted mutagenesis kits
GATA −408	TCC TTG GGG TCT CCG GTA CCC CCA CCA GGG GTG G	A
SF-1 −218	GGG GAT GGC CCT GGT ACC CGG CAT GTT GAC ACA TC	B
	GAT GTG TCA ACA TGC CGG GTA CCA GGG CCA TCC CC
AP1 −203	CAA GGA CAG CAT GTG CGC GCA CAG GCC CAG CTC T	A
GATA −168	GCT CTA TCA CTG GGG AGG GGG TAC CGC TGC CAG GGA CAG	B
	CTG TCC CTG GCA GCG GTA CCC CCT CCC CAG TGA TAG AGC
SOX9/8 −141	ACA GAA AGG GCT GGT ACC GAA GGC CAC TCT G	B
	CAG AGT GGC CTT CGG TAC CAG CCC TTT CTG T
SF-1 −92	CGG GCA CTG TCC CGC GCG CAC GCG GCA GAG GAG	B
	CTC CTC TGC CGC GTG CGC GCG GGA CAG TGC CCG
GATA −74	AGG TCG CGG CAG AGG GTA CCG GGG TCT GTC CTG C	B

A, Altered Sites II in vitro mutagenesis systems kit; B, QuikChange II XL site-directed mutagenesis kit.