Fig. 5.

A, GST-TRα or GST-0 fusion proteins were incubated with S³⁵-labeled Aurora kinase B, and pull-down assays were performed as described (32). Where indicated, T3 (50 nm) was added to the binding assay. B, Endogenous Aurora kinase B was immunoprecipitated from GH4C1 cells in the presence or absence of 5 nm T3 and incubated with histone H3 in kinase buffer with unlabeled ATP in the presence or absence of T3. Kinase activity was evaluated by measuring H3Ser10ph levels by Western blotting with a specific antibody. Coimmunoprecipitated Aurora kinase B and TR were also detected in Western blot assays. The quantification from three different experiments is shown in the graph.