Fig. 6.

Aurora kinase B controls T3-induced transcriptional activity. A, GH4C1 cells were transiently transfected with the TRE-containing DR4-TKLUC reporter plasmid. ZM447439 treatment inhibits T3-induced transactivation. B, GH mRNA expression in GH4C1 cells, analyzed by QRT-PCR 8 h after T3 treatment in the presence or absence of ZM447439. C, A reporter plasmid containing a consensus TRE (DR4-TKLUC) was cotransfected in HEK293T cells together with expression plasmids for TRα and increased amounts of Aurora kinase B. D, Expression vectors for Aurora kinase B (50 ng) or a kinase-dead mutant (K109R, 50ng) were cotransfected with the reporter plasmid in HEK293T cells. Reporter activity was determined after 36 h in the presence or absence of T3. E, ChIP assays with the antibodies indicated in GH4C1 cells maintained for 15 min in the presence or absence of T3 (left). Quantitative PCR was performed with primers flanking the TRE sequence in the GH promoter. Bars represent the mean ± sd (n > 3, right).