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The Nr4a genes are rapidly and transiently induced after 30–60 min NDP-MSH treatment. Total RNA from 3T3-L1 adipocytes treated with 10 nm NDP-MSH from 15 min to 8 h were analyzed by qPCR for the expression of Nr4a1/Nur77 (A), Nr4a2/Nurr1 (B), and Nr4a3/Nor-1 (C). Results were normalized against 36b4 at each time point and expressed as mean ± sem (n = 3). Statistical significance was calculated using the Student's unpaired t test, where *, P < 0.05; **, P < 0.01; ***, P < 0.001.
