Fig. 5.

The NDP-MSH induction of the Nr4a subgroup is concomitant with significant differential expression genes involved in metabolism. Total RNA from 3T3-L1 adipocytes treated with 10 nM NDP-MSH from 15 min to 8 h were analyzed by qPCR for the expression of Il6 (A), Cox2/Ptgs2 (B), Pck1 (C), and Pdk4 (D). Results were normalized against 36b4 at each time point and expressed as mean ± sem (n = 3). E, IL-6 secretion to medium (pictograms per milliliter). Medium from 3T3-L1 adipocytes treated for 2 h with 10 nm NDP-MSH and/or vehicle was collected and measured. Results are mean ± sem of three independent experiments performed in quadruplet. Statistical significance was calculated using the Student's unpaired t test, where *, P < 0.05; **, P < 0.01; ***, P < 0.001.