Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Nur77 siRNA expression significantly attenuates Nur77 expression. 3T3-L1 cells were transfected with 30 nm Nur77 siRNA or negative siRNA (control) for 48 h and differentiated for a further 1 h. qPCR was used to assay the expression of Nur77/Nr4a1, Nurr1/Nr4a2, and Nor-1/Nr4a3. Results were normalized against 36b4 and expressed as mean ± sem (n = 3). Statistical significance was calculated using the Student's unpaired t test, where **, P < 0.01 and ***, P < 0.001.
